ViraDuctin™ Lentivirus Transduction Kit

The efficiency of lentivirus transduction is typically

low. Additives such as Polybrene^® can boost trans-                  700

duction efficiencies, but even then only a small frac-                600

tion of lentiviral vectors can transduce many target

VIRAL EXPRESSION

Polybrene

cell lines.

Our ViraDuctin™ Lentivirus Transduction Kit provides

superior transduction efficiencies in a variety of cell

lines, even when compared to transductions in the

presence of Polybrene^®. The ViraDuctin™ system is

ideal for primary cells and stem cells.

•   Higher Transduction Efficiency: 2-6x higher

in many cell lines compared to Polybrene

•   More Robust: Useful for transduction of non-

500

400

300

200

100

0

NIH3T3    HeLa       293

HT-

1080

ViraDuctin™

permissive cells, including primary cells and

stem cells

Polybrene is a registered trademark of Abbott Laboratories.

Transduction Efficiencies in Various Cell Lines.  NIH3T3

cells, HeLa cells, our own 293AD cells (page 36) and HT-1080

cells were each seeded at 50,000 cells/well in a 24-well plate

overnight. Cells were infected with GFP lentivirus for 48 hours in

the presence of Polybrene® or ViraDuctin™ Lentivirus Transduc-

tion Kit. For each cell line, fluorescence levels using the Vira-

Ductin™ system are depicted relative to a normalized fluores-

cence of 100 for Polybrene^®.

Transduction of 293AD and HT-1080 Cells.  293AD cells (p. 36) and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well

plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of no additive (left), Polybrene^® (middle) or the Vira-

Ductin™ reagent cocktail (right).

Product Name

ViraDuctin™ Lentivirus Transduction Kit 

Size*

40 Transductions

200 Transductions

Catalog Number

LTV-200

LTV-201

*Based on a 24-well plate. Can also be used with 96-well, 12-well or 6-well plates, as well as 60mm or 100mm dishes. See product insert.