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CCL-248 T84 人結(jié)腸癌細(xì)胞

  • 產(chǎn)品/服務(wù):CCL-248 T84 人結(jié)腸癌細(xì)胞
  • 品 牌:上海復(fù)祥生物
  • 單 價(jià):面議 
  • 更新日期:2024-09-04
  • 有效期至:長(zhǎng)期有效
  • 瀏覽次數(shù):1639
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CCL-248 T84 人結(jié)腸癌細(xì)胞 的詳細(xì)介紹CCL-248 T84 人結(jié)腸癌細(xì)胞 ATCC Number: CCL-248...

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CCL-248 T84 人結(jié)腸癌細(xì)胞 的詳細(xì)介紹
CCL-248 T84 人結(jié)腸癌細(xì)胞
ATCC® Number: CCL-248? Price: $264.00
Designations: T84
Depositors: H Masui
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
CCL-248 T84 人結(jié)腸癌細(xì)胞
Source: Organ: colon
Disease: colorectal carcinoma
Derived from metastatic site: lung
Cellular Products: carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-248 T84 人結(jié)腸癌細(xì)胞
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Receptors: neurotransmitter, expressed
peptide hormone, expressed
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8,11
vWA: 17,18
Cytogenetic Analysis: The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1
Age: 72 years
Gender: male
Comments: The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice. [26072]
The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice. [26072]
After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. [1155]
They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport. [1155]
This line exhibits tight junctions, and desmosomes between adjacent cells. [1155]
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The cells should be maintained at high density (at least 1/4 confluency).
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 1
26072: Murakami H, Masui H. Hormonal control of human colon carcinoma cell growth in serum-free medium. Proc. Natl. Acad. Sci. USA 77: 3464-3468, 1980. PubMed:
32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed:

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人胰腺癌細(xì)胞 Panc 10.05 CRL-2547
人肝癌細(xì)胞 SNU-398 CRL-2233
人成神經(jīng)瘤細(xì)胞-骨髓 SK-N-DZ CRL-2149
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人黑色素瘤細(xì)胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細(xì)胞 HUV-EC-C CRL-1730
人黑素瘤細(xì)胞 WM-266-4 CRL-1676
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ROS1728 大鼠成骨細(xì)胞
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CCL-127 IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞
HTB-79 Capan-2 人胰腺癌細(xì)胞
BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞
CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
CRL-1715 TM4 正常小鼠睪丸細(xì)胞
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BRL 大鼠肝細(xì)胞
RSC96大鼠雪旺細(xì)胞
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HLF 人肺成纖維樣細(xì)胞
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293A 人胚腎細(xì)胞
RK-13 兔腎細(xì)胞系CCL-248 T84 人結(jié)腸癌細(xì)胞CCL-248 T84 人結(jié)腸癌細(xì)胞CCL-248 T84 人結(jié)腸癌細(xì)胞CCL-248 T84 人結(jié)腸癌細(xì)胞
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